粳稻苗期长根突变体lr1的形态鉴定和基因定位

doi:10.3969/j.issn.1001-7216.2014.02.002

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 119-124.DOI: 10.3969/j.issn.1001-7216.2014.02.002

粳稻苗期长根突变体lr1的形态鉴定和基因定位

廖莉¹,章骁劼¹,吴建国²,石春海^{1, *}

¹浙江大学农业与生物技术学院农学系，杭州 310058；²浙江农林大学农业与食品科学学院，浙江临安 311300；

收稿日期:2013-10-16 修回日期:2013-10-22 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 石春海1, *
基金资助:
浙江省重大科技攻关专项（2012C129012）；浙江省自然科学基金重点项目（Z3100089）；浙江省科技厅创新团队项目（2010R50024）；教育部创新团队项目（IRT1185）。

Morphological Identification and Gene Mapping of a Seedling Long Root Mutant (lr1) in japonica Rice

LIAO Li¹, ZHANG Xiaojie¹, WU Jianguo², SHI Chunhai ^1,*

¹Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; ²College of Agriculture and Food Science, Zhejiang A&F University, Lin’an 311300, China;

Received:2013-10-16 Revised:2013-10-22 Online:2014-03-10 Published:2014-03-10
Contact: SHI Chunhai1,*

摘要/Abstract

摘要： 通过甲基磺酸乙酯诱导粳稻品种日本晴，筛选到一个能稳定遗传的苗期长根突变体 lr1 （long root 1）。在发芽后2、4、6、10和15 d， lr1 的主根长度分别比日本晴野生型主根长1.52、1.93、3.93、6.81和6.77 cm，根冠比也显著大于野生型。遗传分析结果表明该基因受一对隐性单基因控制。利用SSR分子标记将该突变基因 LR1 定位于第10染色体长臂上，位于标记RM304和RM7300之间，遗传距离分别为20 cM和3.5 cM。

关键词: 水稻, 长根, 形态鉴定, 基因定位

Abstract: A seedling long root mutant named lr1 (long root 1) was isolated from rice cultivar Nipponbare by mutagenesis with ethyl methanesulfonate (EMS) and the long root trait can be stably inherited. The main root in lr1 mutant was 1.52, 1.93, 3.93, 6.81 and 6.77 cm longer than those of wild type at 2, 4, 6, 10 and 15day after germination, respectively, and the rootshoot ratio of lr1 mutant was also significantly greater than that in the seedling of Nipponbare. Genetic analysis indicated that the seedling long root mutant phenotype was controlled by a single recessive gene. The LR1 gene was located between the SSR markers RM304 and RM7300 on chromosome 10, and the genetic distance is 20 cM and 3.5 cM, respectively.

Key words: rice, long root, morphological identification, gene mapping

中图分类号:

Q343.5
Q786

廖莉1 ,章骁劼1 ,吴建国2 ,石春海1, *. 粳稻苗期长根突变体lr1的形态鉴定和基因定位[J]. 中国水稻科学, 2014, 28(2): 119-124.

LIAO Li1, ZHANG Xiaojie1, WU Jianguo2, SHI Chunhai1,*. Morphological Identification and Gene Mapping of a Seedling Long Root Mutant (lr1) in japonica Rice[J]. Chinese Journal of Rice Science, 2014, 28(2): 119-124.

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粳稻苗期长根突变体lr1的形态鉴定和基因定位

Morphological Identification and Gene Mapping of a Seedling Long Root Mutant (lr1) in japonica Rice

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